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NA11099 DNA from Somatic cell hybrid

Description:

CHROMOSOME 16 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
CHROMOSOME X REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS

Affected:

No Data

Sex:

No Data

Age:

No Data

  • Overview
  • Characterizations
  • Phenotypic Data
  • Publications
  • External Links

Overview

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Repository NIGMS Human Genetic Cell Repository
Subcollection Hybrids
Quantity 0.050mg
Quantitation Method Please see our FAQ
Cell Type Somatic cell hybrid
Transformant Untransformed
Sample Source DNA from Somatic cell hybrid
Confirmation Karyotypic analysis and Southern blot hybridization
ISCN Human/mouse somatic cell hybrid retaining human der(X)t(X;16) chromosome
Remarks Line CY2; produced by the fusion of human fibro, GM03884, 46,X,t(X;16)(q26; q24), with APRT- & HPRT- deficient mouse A9 cells; selected in alanosine-adenine medium; grown in AAT medium; retains human der(X)t(X;16) in 100% of cells

Characterizations

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IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis
 
GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for Xp22.32 (also Yp11.3), MIC2, and gave a negative result with a probe for Xq28, DXS15. PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for Xq26, HPRT and Xq28, G6PD, and gave positive results with primers for Xp11.21, ALAS2, Xp11.3, DXS1003, Xp22.32, STS, Xq12, DXS453, Xq13.3, PGK1, and Xq21.3, DXS3.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 16 DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for 16pterp13, D16S36, and 16q13q22.1, MT2. PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 16p13.3, HBA, 16q22.1, LCAT, and 16q23.2, D16S266, and gave a positive result with a primer for 16q24.3, PRSM1.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 17 DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 17q21.3q23, MPO.
 

Phenotypic Data

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Remarks Line CY2; produced by the fusion of human fibro, GM03884, 46,X,t(X;16)(q26; q24), with APRT- & HPRT- deficient mouse A9 cells; selected in alanosine-adenine medium; grown in AAT medium; retains human der(X)t(X;16) in 100% of cells

Publications

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Pilia G, Hughes-Benzie RM, MacKenzie A, Baybayan P, Chen EY, Huber R, Neri G, Cao A, Forabosco A, Schlessinger D, Mutations in GPC3, a glypican gene, cause the Simpson-Golabi-Behmel overgrowth syndrome [see comments] Nat Genet12:241-7 1996
PubMed ID: 8589713
 
Oaks MK, Hanson JP Jr, O'Malley DP, Molecular cytogenetic mapping of the human melanoma antigen (MAGE) gene family to chromosome region Xq27-qter: implications for MAGE immunotherapy. Cancer Res54:1627-9 1994
PubMed ID: 8137270
 
Callen DF, Doggett NA, Stallings RL, Chen LZ, Whitmore SA, Lane SA, Nancarrow JK, Apostolou S, Thompson AD, Lapsys NM, et al, High-resolution cytogenetic-based physical map of human chromosome 16. Genomics13:1178-85 1992
PubMed ID: 1505951
 
Callen DF, Baker E, Eyre HJ, Lane SA, An expanded mouse-human hybrid cell panel for mapping human chromosome 16. Ann Genet33:190-5 1990
PubMed ID: 2095701
 
Callen DF, Hyland VJ, Baker EG, Fratini A, Simmers RN, Mulley JC, Sutherland GR, Fine mapping of gene probes and anonymous DNA fragments to the long arm of chromosome 16. Genomics2:144-53 1988
PubMed ID: 2900808
 
Callen DF, A mouse-human hybrid cell panel for mapping human chromosome 16. Ann Genet29:235-9 1986
PubMed ID: 3495225

External Links

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dbSNP dbSNP ID: 11481
Pricing
International/Commercial/For-profit:
$449.00USD
U.S. Academic/Non-profit/Government:
$263.00USD
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