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NA14971 DNA from Somatic cell hybrid

Description:

CHROMOSOME 11 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
ADDITIONAL DELETION/DERIVATIVE CHROMOSOME 16 SOMATIC CELL HYBRIDS

Affected:

No Data

Sex:

No Data

Age:

No Data

  • Overview
  • Characterizations
  • Phenotypic Data
  • External Links

Overview

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Repository NIGMS Human Genetic Cell Repository
Subcollection Hybrids
Quantity 0.050mg
Quantitation Method Please see our FAQ
Cell Type Somatic cell hybrid
Transformant Untransformed
Sample Source DNA from Somatic cell hybrid
Confirmation Karyotypic analysis and In situ hybridization
ISCN Human/mouse somatic cell hybrid retaining a human der(16)t(11;16)(q21;p12.3) in 56% of cells
Remarks Line CY11; produced by the fusion of human fibros, GM03005, 46,XX,t(11;16)(q13;p11), with HPRT- and APRT-deficient mouse A9 cells; culture is grown in medium containing adenine and azaserine; retains a human der(16)t(11;16)(q21;p12.3) in 56% of cells; this hybrid tends to form human/rodent translocations and users who elect to use the culture rather than the DNA sample should monitor the culture for retention of the der(16)

Characterizations

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IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis
 

Phenotypic Data

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Remarks Line CY11; produced by the fusion of human fibros, GM03005, 46,XX,t(11;16)(q13;p11), with HPRT- and APRT-deficient mouse A9 cells; culture is grown in medium containing adenine and azaserine; retains a human der(16)t(11;16)(q21;p12.3) in 56% of cells; this hybrid tends to form human/rodent translocations and users who elect to use the culture rather than the DNA sample should monitor the culture for retention of the der(16)

External Links

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dbSNP dbSNP ID: 12230
Pricing
International/Commercial/For-profit:
$449.00USD
U.S. Academic/Non-profit/Government:
$263.00USD
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