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NA11939 DNA from Somatic cell hybrid

Description:

CHROMOSOME 11 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS

Affected:

No Data

Sex:

No Data

Age:

No Data

  • Overview
  • Characterizations
  • Phenotypic Data
  • Publications
  • External Links

Overview

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Repository NIGMS Human Genetic Cell Repository
Subcollection Hybrids
Quantity 0.050mg
Quantitation Method Please see our FAQ
Cell Type Somatic cell hybrid
Transformant Untransformed
Sample Source DNA from Somatic cell hybrid
Confirmation Clinical summary/Case history
ISCN Mouse/human hybrid retaining human der(16)t(11;16), #8, 9, 13, 14, 15, 17, 19, 20, 21, & 22
Remarks Line 16TX2; product of the fusion of human lymphoblasts, 46,XX,t(11;16)(p11.2; p11.1), GM06316A, with HPRT-deficient mouse myeloma P3/NS-1 cells; grown without selection; retains human der(16)t(11;16) in 76% of cells and other human chromosomes

Characterizations

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IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 1 DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 1p36, PND.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 11 DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 11p13, CAT, and gave a negative result with a probe for 11q23.3, ETS1. PCR analysis of DNA from this somatic cell hybrid gave a positive result with a primer for 11p13, CAT, and gave a negative result with a primer for 11q21-q22, CLG.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 13 DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 13q34, D13S3.
 
GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 16 DNA from this somatic cell hybrid gave a positive result in Southern blot hybridization analysis with a probe for 16q13q22.1, MT2, and gave a weakly positive result with a probe for 16pterp13, D16S36.
 

Phenotypic Data

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Remarks Line 16TX2; product of the fusion of human lymphoblasts, 46,XX,t(11;16)(p11.2; p11.1), GM06316A, with HPRT-deficient mouse myeloma P3/NS-1 cells; grown without selection; retains human der(16)t(11;16) in 76% of cells and other human chromosomes

Publications

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Leek J, Lench N, Maraj B, Bailey A, Carr IM, Andersen S, Cross J, Whelan P, MacLennan KA, Meredith DM, Prostate-specific membrane antigen: evidence for the existence of a second related human gene British journal of cancer72:583-8 1995
PubMed ID: 7669565
 
Junien C, van Heyningen V, Evans G, Little P, Mannens M, Report of the second chromosome 11 workshop. Genomics12:620-5 1992
PubMed ID: 1348491
 
Seawright A, Fletcher JM, Fantes JA, Morrison H, Porteous DJ, Li SS, Hastie ND, Van Heyningen V, Analysis of WAGR deletions and related translocations with gene- specific DNA probes, using FACS-selected cell hybrids. Somat Cell Mol Genet14:21-30 1988
PubMed ID: 2829363
 
Spurr NK, Gough AC, Gosden J, Rout D, Porteous DJ, van Heyningen V, Docherty AJ, Restriction fragment length polymorphism analysis and assignment of the metalloproteinases stromelysin and collagenase to the long arm of chromosome 11. Genomics2:119-27 1988
PubMed ID: 2900807

External Links

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dbSNP dbSNP ID: 11660
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