GM08688
LCL from B-Lymphocyte
Description:
BURKITT LYMPHOMA; BL
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Heritable Diseases
Chromosome Abnormalities
dbGaP |
| Class |
Heritable Cancer Syndromes and other Cancers |
| Alternate IDs |
GM17349 [BURKITT LYMPHOMA; BL] |
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Biopsy Source
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Peripheral vein
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Cell Type
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B-Lymphocyte
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Tissue Type
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Blood
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Transformant
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Epstein-Barr Virus
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Sample Source
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LCL from B-Lymphocyte
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Race
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Black/African American
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Ethnicity
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AFRICAN
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XY,t(8;22)(q24;q11),der(11)t(11;16)(q23.2;q21)[43] / 47,XY,+7, +der(1)t(1;16)(p13;p12),-16,t(8;22),dic(10;16)(p15;p12),der(11)t(11;16)(q23.2;q21)[17].ish t(8;22)(myc+;myc+)
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Cytogenetics |
Chromosome 22: TRANSLOCATION Breakpoint 22q11 t(8;22)22q11 |
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Chromosome 8: TRANSLOCATION Breakpoint 8q24 t(8;22)8q24 |
| Remarks |
Line IARC/LY47; Ugandan; 46,XY,t(8;22) (8pter>8q24::22q11>22qter;22pter>22q11:: 8q24>8qter); 68% of cells were 47,XY,+7, +der(1)t(1;16),-16,t(8;22),t(10;16) |
| Bernheim A, Berger R, Lenoir G, Cytogenetic studies on African Burkitt's lymphoma cell lines: t(8;14), t(2;8) and t(8;22) translocations. Cancer Genet Cytogenet3:307-15 1981 |
| PubMed ID: 7260888 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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